Optimization of Nutrient Medium Composition for Alpha- Amylase Biosynthesis by Aspergillus Oryzae Pp
نویسندگان
چکیده
In the production of industrial enzymes, filamentous fungi are attractive as cell factories because they can secrete large amounts of protein into the medium. Many important industrial enzymes such as amylases, lipases and cellulase are produced by fermentation of filamentous fungi. Aspergillus oryzae is well-known filamentous producer of alpha amylase and has GRAS status. In the present study an improvement of alpha amylase activity from Asprgillus oryzae PP was achieved by changes in medium components. Optimization of the nutrient composition of the fermentation medium was carried out by the method of complete factorial planning of experiment Box-Wilson plan.
منابع مشابه
Production of amylase in liquid culture by a strain of Aspergillus oryzae.
The effect of different media and pH on the formation of amylase by Aspergillus oryzae EI 212 is described. Depending upon the composition of the medium and growth conditions, the fungus was found to secrete alpha- or beta-amylase, or both. Some of the properties of the partially purified alpha-amylase were found to be different from alpha-amylases from other sources.
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